Safra kesesi kanserinde Helikobakter 16SrDNA ve üreaz gen analizi
Analysis of Helicobacter 16SrDNA and urease gene in gallbladder carcinoma
Özet
Giris ve amaç: Kronik kolesistit hastalarinin, safra ve rezeke safra keselerinde, Helikobakter türlerinin varligi gösterilmistir. Bu veriler, Helikobakter türlerinin safra kesesi kanseri ile iliskisini akla getirmektedir. Bu çalismada, safra kesesi kanserinde Helikobakter DNA analizi amaçlanmi stir. Gereç ve yöntem: 1995-2000 yillari arasinda EÜTF Patoloji laboratuvari nda, rezeke safra keselerinde kanser saptanan 31 olgu çalismaya alinmistir. Bu hastalarin parafin bloklarindan alinan kesitlerde, polimeraz zincir reaksiyonu (PCR) ile Helikobakter 16SrDNA ve üreaz gen analizi yapilmistir. Kontrol grubu olarak karaciger transplantasyon donörü 10 olgunun normal safra kesesi dokulari kullanilmistir. Bulgular: Safra kesesi adenokarsinomu olan tek bir olgunun tümöre komsu, tümörsüz safra kesesi dokusunda üreaz geni pozitif bulunmustur. Safra kesesi kanserli diger olgularin, tümörlü ve tümörsüz dokularinda ve kontrol grubunun normal safra kesesi dokularinda Helikobakter 16SrDNA ve üreaz geni saptanmamistir. Sonuç: Bu bulgular, safra kesesi kanseri ile Helikobakter türleri arasinda öngörülen iliskiyi desteklememektedir.
Abstract
Background/aim: The presence of Helicobacter species has been shown in bile and the resected gallbladder of patients with chronic cholecystitis. Therefore, an association between Helicobacter and gallbladder carcinoma might be proposed. The aim of this study was to analyze the presence of Helicobacter DNA in gallbladder carcinoma. Materials and methods: Thirty-one patients diagnosed as gallbladder carcinoma in the EÜTF Pathology Laboratory between 1995-2000 were included in the study. Sections were taken from their paraffin-embedded gallbladder tissue. Polymerase chain reaction (PCR) for Helicobacter 16SrDNA and urease gene was performed. Normal gallbladder tissues of 10 liver transplantation donors were used as controls. Results: Helicobacter urease gene was found positive only in one patient with gallbladder adenocarcinoma. Helicobacter 16SrDNA and urease gene were both negative in all the gallbladder tissues of the other cases with gallbladder carcinoma and of the control group. Conclusion: Thus, our data did not support the proposed association between Helicobacter species and gallbladder carcinoma.
