Mide kanserinde E-cadherin genindeki heterozigosite kaybinin sıklığı
Frequency of loss of heterozygosity of E-cadherin gene in gastric cancer
Özet
Giriş ve amaç: Ülkemizde mide kanseri gastrointestinal kanserler içerisinde ilk sirada yer alir. E-cadherin bir adezyon molekülüdür ve doku bütünlügünün korunmasi, tümör invazyonu ve tümör metastazi gibi birçok olayda rol alir. Çalışmamızda bölgemizdeki mide kanserlerinde E-cadherin geninde heterozigosite kaybi (LOH) sikligini araştırdık. Gereç ve yöntem: karın ağrısı, bulanti-kusma, hematemez ve kilo kaybı şikayetleri nedeniyle müracaat eden ve mide kanseri tanısı konulan 25 hasta dahil edildi. Alinan materyallerin histopatolojik inceleme sonucu kanser olduğu dogrulandi. Örneklerden usulüne uygun olarak DNA izolasyonu yapildi. Elde edilen DNA örneklerinden PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) teknigi ile E-cadherin genindeki LOH düzeyine bakildi. Bulgular: Lauren siniflamasina göre Diffüz tip vakalardan bir tanesinde LOH tespit edildi (%33,3). Intestinal tip vakalardan 22 hastamizda LOH tespit edilemedi. Tespit edilen LOH 25 vaka göz önüne alındıginda %4 oraninda idi. Sonuç: LOH, sadece diffüz tip mide kanseri materyallerinde tespit edildi. Bundan dolayi diffüz tip mide kanseri gelisimi ile LOH kaybi arasında ilişki olabilir.
Abstract
Background/aim: Gastric cancer is the most frequent malignancy in gastrointestinal cancers in our country. E-cadherin is an adhesion molecule which plays a role in many events like the preservation of tissue integrity, tumor invasion and tumor metastasis. The aim of this study was to determine the frequency of loss of heterozygosity (LOH) of E-cadherin gene in gastric cancer in our region. Materials and methods: 25 patients who admitted with the complaints of abdominal pain, nausea and vomiting, hematemesis and weight loss and were diagnosed as gastric cancer were enrolled in this study. Histopathological examination of biopsy specimens confirmed the clinical diagnosis of gastric cancer. DNA isolation was made from the specimens, and LOH in E-cadherin gene was detected by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technique. Results: Of diffuse type gastric cancer cases (n=3) according to Lauren Classification, LOH was determined in 1 patient (33.3%). No LOH was determined in 22 patients with intestinal type gastric cancer. Thus, LOH frequency was only 4% among all cases. Conclusion: As LOH was determined only in a diffuse type gastric cancer biopsy material, there may be a relation between them.
