Giris ve Amaç: Bu çalismanin amaci metotreksata bagli oksidatif ince barsak hasarinda leflunomid tedavisinin etkisini arastirmaktir. Gereç ve Yöntem: 40 adet rat randomize olarak dört gruba ayrilmistir. Metotreksata bagli ince barsak hasari 20 mg/kg metotreksatin tek doz intraperitoneal injeksiyonu ile olusturulmustur. Indüksiyon sonrasi leflunomid 10 mg/kg/gün dozunda intra gastrik olarak 5 gün boyunca verilmistir. Ince barsak dokulari, 6. gün superoksit dismutaz aktivitesi, myeloperoksidaz aktivitesi, glutatyon ve malondialdehit düzeylerinin ölçümü için homojenize edilmistir. Bulgular: Leflunomid tedavisi doku myeloperoksidaz aktivitesi ve malondialdehit düzeylerini anlamli ölçüde azaltmistir (Sirasiyla 3.4±0.7, 1.9±0,4 p<0.05 ve 231±199, 94±80 p<0.05). Leflunomid tedavisi doku glutatyon düzeyleri ve süperoksit dizsmutaz aktivitesindeki azalmayi anlamli ölçüde iyilestirmistir (Sirasiyla 21±0.5, 25±3 p<0.05 ve 22±0.6, 25±2 p<0.05). Sonuç: Leflunomid tedavisi metotreksata bagli ince barsak hasarindaki oksidatif parametreleri düzeltmektedir.
Background and Aims: The aim of this study was to investigate the effects of leflunomide treatment in methotrexate-induced oxidative small intestinal injury in rats. Materials and Methods: Forty rats were randomly divided into 4 groups. Methotrexate-induced intestinal injury was induced by single dose intraperitoneal injection of 20 mg/kg methotrexate. After induction, leflunomide (10 mg/kg/day) was administered intragastrically for 5 consecutive days. On the sixth day, homogenized small intestine tissues were examined for superoxide dismutase activity, myeloperoxidase activity, and glutathione and malondialdehyde levels. Results: Leflunomide treatment significantly decreased tissue myeloperoxidase activity and malondialdehyde levels (3.4±0.7, 1.9±0.4, p<0.05 and 231±199, 94±80, p<0.05, respectively). Leflunomide treatment significantly improved the decreased tissue levels of glutathione and superoxide dismutase activity (21±0.5, 25±3, p<0.05 and 22±0.6, 25±2, p<0.05, respectively). Conclusions: Leflunomide treatment ameliorated oxidative parameters of methotrexateinduced small intestinal injury.