Ülkemizdeki hepatit D virus genotipi
Hepatitis D virus genotype in our country
Özet
Bu çalismanin amaci; hepatitis D virusu ile infekte kronik hepatitli hastalarda genotip tayini yapmaktir. Çalisma kapsamina 25 hasta alinmistir. HDV-RNA Boom metodu, hepatitis D virusu genotip tayini ise RT-PCR ve RFLP metodu ile çalisilmistir. 22 hastada genotip I saptanmistir. 3 hastada genotip tayini yapilamamistir. Sonuç olarak; ülkemizdeki hepatitis D virusu, genotip I’dir.
Abstract
The aim of this study was to identify hepatitis D virus genotypes in our chronic hepatitis D patients. Twenty-five patients with hepatitis D virus infection were enrolled in this study. HDV-RNA was extracted from the serum by Boom methods. Hepatitis D virus genotype was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) methods. Genotype I was found in 22 patients, and could not be determined in the remaining 3 patients. In conclusion, genotype I is the dominant hepatitis D virus genotype in our country.
