Kronik hepatit B’li hastalarda karaciger dokusu ICAM-1 ve ICAM-3 düzeyleri
The level of ICAM-1 and ICAM-3 in liver tissue in patients with chronic hepatitis B
Özet
Giris ve amaç: Hücrelerarasi adezyon molekülü (ICAM)-1 esas olarak lökositlerin inflamasyonun oldugu dokuya ait hücrelerle adezyonundan, ICAM-3 ise daha çok lenfositlerin immün sistemin diger hücreleriyle adezyonundan sorumludur. Yetersiz dentritik hücre fonksiyonu hepatit B virüs enfeksiyonunun kroniklesmesinden sorumlu tutulmakta olup ICAM-3 dentritik hücrelerin lenfositlerle adezyonundan asil sorumlu moleküldür. Bu çalismanin amaci kronik hepatit B’ li hastalarda ICAM- 1 ve ICAM-3 düzeylerini belirlemektir. Gereç ve yöntem: Çalismaya HBs Ag pozitif, histopatolojik olarak kronik hepatit tanisi konulmus 31 olgu ile viral ve otoimmün markerlari negatif olup karaciger biyopsisi normal olarak degerlendirimis 24 olgu alindi. Olgularin biyokimyasal ve serolojik tetkikleri yapildi, karaciger dokusunda hepatosit, sinüzoid endotel ve lenfositlerinde ICAM-1 ve ICAM-3 düzeyleri immünhistokimyasal metodla bakildi, sonuçlar boyanma yogunluguna göre 0-3 araliginda skorlanarak degerlendirildi. Bulgular: Kronik hepatitli olgularada hepatosit ICAM-1 skorunun (1.48±0.9) kontrol grubundan (0.66±0.4) yüksek oldugu görülürken (p<0.001), bu olgularda sinüzoid endotel ve lenfositlerinde ICAM-3 düzeyinin kontrol grubundan yüksek olmadigi görüldü (p>0.05). Sonuç: Kronik hepatitli olgularda karacigerde lenfositik ICAM-3 skorunda beklendigi gibi artis olmayip, hastaligin fizyopatolojisinde ICAM-3’ le iliskili fonksiyonlarin, bagli olarak dentritik hücre-T lenfosit adezyonunun yetersizliginin rolü olabilecegi düsünülebilir.
Abstract
Background and aims: Whereas intercellular adhesion molecule (ICAM)-1 is primarily responsible for the adhesion between the leucocytes and cells of inflamatory tissue, ICAM-3 is responsible for the adhesion between lymphocytes and other cells of the immune system. Chronic Hepatitis B infection is caused by inadequate dendritic cell function and ICAM-3 is the primary molecule causing the adhesion between dendritic cells and lymphocytes. The aim of this study was to determine the ICAM-1 and ICAM-3 expression of patients with chronic hepatitis B infections. Materials and methods: Thirty one patients with HBs-Ag positivity and histopathologically diagnosed as chronic hepatitis and 20 cases with normally liver biopsy results entered the study. Biochemical and serological evaluation of cases was made; ICAM-1 and ICAM-3 expression were determined by immunohistochemical methods in the hepatocytes, sinusoidal endothelium and lymphocytes of the liver tissues. Results were evaluated according to staining density with a score of 0-3. Results: Hepatocyte ICAM-1 expression (1.48 ± 0.9) was higher in chronic hepatitis cases (p<0.001) than the control group (0.66 ± 0.4) but there was no significant difference in ICAM-3 levels in sinusoidal endothelium and lymphocytes (p>0.005). Conclusions: As there was not such an increase in liver lymphocyte ICAM-3 as expected in chro-nic hepatitis patiens, the suggests that ICAM-3 related functions and inadequate dendritic cell-T lymphocyte adhesion may play a role in the pathophysiology of this disease.
