Assidik sivi hücre sayiminda otomatik yöntem yeterince güvenilir bir seçenek midir?
Is automatic method sufficiently reliable for ascitic fluid cell count?
- Ana Sayfa
- Sayılar
- Assidik sivi hücre sayiminda otomatik yöntem yeterince güvenilir bir seçenek midir?...
Özet
Amaç: Assit sivisi hücre sayimi assit sivi analizinde rutin kullanilan bir yöntemdir. Çalismamizda assit sivisi hücre sayiminda kullanilan iki yöntemi (Hemogram cihazi kullanilarak sayimin yapildigi otomatik yöntem ve Thoma lami kullanilarak yapilan standart manuel hemositometri yöntemi) karsilastirmayi amaçladik. Gereç ve yöntem: Klinigimize assit nedeniyle yatirilan hastalar çalismaya dahil edildi. Her hastadan assit sivisi alindi ve bu assit sivisindan hem otomatik hem de Thoma lam yöntemiyle hücre sayimi yapildi. Bulgular: Yaslari 19-63 arasinda degisen 20’si erkek, 6’si kadin toplam 26 hastanin 44 assit örnegi incelendi. 23’ünde otomatik ölçümle, 14’ünde de Thoma lam yöntemiyle beyaz küre esik degeri olan 500 hücre /mm3 düzeyi asilmis bulundu. Buna göre otomatik ölçümle esik degerin asildigi 9 hastada Thoma lam yönteminde sonuç normal sinirlarda bulundu. Bu 9 hastanin hiçbirinde assit sivisi kültüründe üreme saptanmadi. Sonuç: Otomatik ölçümlerde "overdiagnosis" olabilecegini düsünmekteyiz. Daha genis hasta gruplariyla çalismanin dogrulanmasi gerekmektedir.
Abstract
Background and aims: Ascitic fluid cell count is used routinely in analysis of ascitic fluid. In this study, two methods used in ascitic fluid cell count were compared (automatic method in which a hemogram device is used and standard manual hemocytometer count method in which a Thoma lam is used). Materials and methods: Twenty-six patients (20 men, six women, age range: 19-63 years) admitted to our clinic with ascites were included in the study and ascites fluid obtained from each patient, following which cell counts were performed by both methods. Results: A total of 44 ascitic fluid samples were examined. The upper limit of normal total White Blood Cell (WBC) count (>500 cell/mm3) was exceeded in 23 samples analyzed by the automatic method and 14 samples by the Thoma lam method. Nine out of 23 patients in whom cut-off levels were exceeded by automatic method had normal results with the Thoma lam method. There was no culture-positive result in any of these nine patients. Conclusions: Overdiagnosis may be obtained by use of the automatic method in evaluating ascitis fluid cell counts and the results of this study indicate that further investigation of this subject should be undertaken.
